Prodigiosin-loaded electrospun nanofibers scaffold for localized treatment of triple negative breast cancer

dc.contributor.author Akpan, U. M.
dc.contributor.author Pellegrini, M.
dc.contributor.author Obayemi, J. D.
dc.contributor.author Ezenwafor, T.
dc.contributor.author Browl, D.
dc.contributor.author Ani, C. J.
dc.contributor.author Yiporo, D.
dc.contributor.author Salifu, A.
dc.contributor.author Dozie-Nwachukwu, S.
dc.contributor.author Odusanya, S.
dc.contributor.author Freeman, J.
dc.contributor.author Soboyejo, W. O.
dc.date.accessioned 2021-01-13T09:53:18Z
dc.date.available 2021-01-13T09:53:18Z
dc.date.issued 2020-09
dc.description For full-text see http://www.sciencedirect.com/science/article/pii/S0928493119335192 en_US
dc.description.abstract Hybrid composite nanofibers, with the potential to enhance cell adhesion while improving sustained drug release profiles, were fabricated by the blend electrospinning of poly(d,l-lactic-co-glycolic acid) (PLGA), gelatin, pluronic F127 and prodigiosin (PG). Scanning Electron Microscopy (SEM) images of the nanofibers revealed diameters of 1.031 ± 0.851 μm and 1.349 ± 1.264 μm, corresponding to PLGA/Ge-PG and PLGA/Ge-F127/Ge, respectively. The Young's moduli were also determined to be 1.446 ± 0.496 kPa and 1.290 ± 0.617 kPa, while the ultimate tensile strengths were 0.440 ± 0.117 kPa and 0.185 ± 0.480 kPa for PLGA/Ge-PG and PLGA/Ge-F127/Ge, respectively. In-vitro drug release profiles showed initial (burst) release for a period of 1 h to be 26.000 ± 0.004% and 16.000 ± 0.015% for PLGA/Ge and PLGA/Ge-F127 nanofibers, respectively. This was followed by 12 h of sustained release, and subsequent slow sustained release of PG from the composite nanofibers. The cumulative release of PG (for three days) was determined to be 82.0 ± 0.1% for PLGA/Ge and 49.7 ± 0.1% for PLGA/Ge-F127 nanofibers. The release exponents (n) show that both nanofibers exhibit diffusion-controlled release by non-Fickian (zeroth order) and quasi-Fickian diffusion in the initial and sustained release regimes, respectively. The suitability of the composite nanofibers for supporting cell proliferation and viability, as well as improving sustained release of the drug were explored. The in-vitro effects of cancer drug (PG) release were also studied on breast cancer cell lines (MCF-7 and MDA-MB-231 cells). The implications of the results are discussed for the potential applications of drug-nanofiber scaffolds as capsules for localized delivery of chemotherapeutic drugs for the treatment of triple negative breast cancer. en_US
dc.identifier.citation U.M. Akpan, M. Pellegrini, J.D. Obayemi, T. Ezenwafor, D. Browl, C.J. Ani, D. Yiporo, A. Salifu, S. Dozie-Nwachukwu, S. Odusanya, J. Freeman, W.O. Soboyejo, Prodigiosin-loaded electrospun nanofibers scaffold for localized treatment of triple negative breast cancer, Materials Science and Engineering: C, Volume 114, 2020, 110976, ISSN 0928-4931, https://doi.org/10.1016/j.msec.2020.110976. (http://www.sciencedirect.com/science/article/pii/S0928493119335192) en_US
dc.identifier.uri http://hdl.handle.net/20.500.11988/599
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.subject breast cancer en_US
dc.subject composite nanofibers en_US
dc.subject localised treatment en_US
dc.title Prodigiosin-loaded electrospun nanofibers scaffold for localized treatment of triple negative breast cancer en_US
dc.type Article en_US
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